| 1. | Analyzing the characteristics of start codon in prokaryote and yeast genomes
原核生物和酵母基因组中起始密码的特征分析 |
| 2. | ( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed
( 2 )在翻译水平上通过pcr扩增的方式在t - pa基因起始密码子处添加了植物翻译起始共有序列aaca ，构建了植物表达载体pbet 。 |
| 3. | 5 ' race analysis indicated that ha 122 transcripts start predominantly in the consensus major late transcription initiation motif rtaag around 47nt upstream of the putative translation start codon with a minor start at position - 89
5 ’ race ( rapidamplificationofcdnaend )分析结果表明ha122基因的转录主要从翻译起始密码子上游第47个碱基起始，该处有一个晚期转录信号taag 。 |
| 4. | By using the method of genome walking , two fragments were cloned from genome walker library after the semi - nested pcr . the result of sequencing the bigger fragment showed that this 706bp sequence contained the atg start codon
通过半巢式的基因步移法，经二轮pcr后，从genomewalker文库分离到2个片段，较大的片段测序表明该706bp序列含有编码ast的起始密码子atg ，其上游序列为启动子序列。 |
| 5. | A late transcriptional motif , ataag , was found at - 50nt upstream of the translational start codon atg , while two tata box was located at - 112nt and - 189nt upstream of atg . a typical poly ( a ) signal was found at 12nt downstream of the translational stop codon
在hel起始密码子atg上游50位有强晚期启动子转录起始信号ataag ，在? 112位和? 189位存在两个tatabox ，但未发现早期转录信号cagt 。 |
| 6. | The start codon is at 84 - 86bp , the stop codon at 1658 - 1661bp . the homologous comparison of the deduced amino acid sequence with other plant epsp synthase proteins shows the identity to those of b . napus , a . thaliana , tomato , n . labacum respectively are 92 % , 91 % , 80 % , 65 %
同源比较该段序列所编码的氨基酸序列可以发现epsp合成酶有较高的保守性，它与欧洲油菜、拟南芥、西红柿、烟草的同源性分别为920 、 91o 、 80o 、 65o 。 |
| 7. | Histochemical gus assay showed that the gus staining was observed only in the mature pollen and germination pollen tube . there is no detectable gus activity in other floral organs , leaves and stems . these results suggested that st901 is a novel pollen - specific gene and the 288bp promoter fragment ( - 297 ? xfrom the translation start codon atg ) is sufficient for pollen - specific expression and os - element regulatory of st901 promoter was possibly concentrated in the region - 297 to - 9
通过对gus酶活性的组织化学定位分析，表明， st901基因启动子驱动gus基因特异地在成熟花粉和花粉管中表达， st901基因具有花粉表达特性；且288bp ( - 297至- 9 ) ( atg定为+ 1 )的启动子区段足以驱动gus基因在花粉中的特异表达， st901基因启动子的花粉顺式表达元件可能位于- 297至- 9之间。 |
